| 质粒名称: | pMuLE Lenti Dest iRFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 62176 |
| 存储实验室: | Ian Frew |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒；MuLE网关目标向量 |
| 筛选标记: | iRFP |
| 载体抗性: | 氯霉素和氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DB3.1 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMuLE Lenti Dest iRFP was a gift from Ian Frew (Addgene plasmid # 62176 ; http://n2t.net/addgene:62176 ; RRID:Addgene_62176) |
| 参考文献部分: | A versatile modular vector system for rapid combinatorial mammalian genetics. Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ. J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. 10.1172/JCI79743 PubMed 25751063 |
买家导航
pMuLE Lenti Dest iRFP载体质粒基本信息
pMuLE Lenti Dest iRFP质粒图谱载体图谱和pMuLE Lenti Dest iRFP载体序列质粒序列多克隆位点信息
pMuLE Lenti Dest iRFP质粒载体简介
pMuLE Lenti Dest iRFP质粒序列载体序列
LOCUS pMuLE Lenti Dest iRFP 9377 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION MuLE (Multiple Lentiviral Expression) Destination vector for use
with pMuLE Entry vectors. Co-expresses iRFP under the control of a
PGK promoter. Compatible with MultiSite Gateway cloning.
ACCESSION .
VERSION .
KEYWORDS pMuLE Lenti Dest iRFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9377)
AUTHORS Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T,
Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK,
Bode-Lesniewska B, Wild PJ, Frew IJ
TITLE A versatile modular vector system for rapid combinatorial mammalian
genetics.
JOURNAL J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
PUBMED 25751063
REFERENCE 2 (bases 1 to 9377)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9377
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 94..327
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 399..520
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(436..455)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 490..509
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 539..674
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 601..620
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(706..725)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(707..725)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(735..752)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(735..751)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(744..766)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 896..1351
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(983..1002)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1193..1214
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 1380..1484
/gene="bla"
/label=AmpR promoter
CDS 1485..2345
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1703..1722)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2516..3104
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3005..3024
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3258..3275
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3392..3413
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3428..3458
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3466..3482
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3471..3493
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 3490..3506
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3490..3506
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 3525..3545
/label=T3
/note="T3 promoter, forward primer"
promoter 3527..3545
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
LTR 3801..3981
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4025..4150
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 4643..4876
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 5060..5104
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 5365..5482
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 5537..5661
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 5686..5716
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 5770..6429
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(5836..5855)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 6771..7076
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 6955..6974
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(7117..7241)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 7272..7860
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7325..7345)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7743..7754)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 7874..8373
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(7909..7932)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 8306..8325
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 8411..8420
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 8417..9367
/codon_start=1
/product="phytochrome-based near-infrared fluorescent
protein, originally termed iRFP (Filonov et al., 2011)"
/label=iRFP713
/note="name was changed to iRFP713 (Shcherbakova and
Verkhusha, 2013)"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
ORIGIN
1 cgcgtatgca tctcgagggc ccggtacctt taagaccaat gacttacaag gcagctgtag
61 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa
121 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
181 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
241 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
301 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
361 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
541 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
601 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
661 cttttttgga ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg
721 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
781 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
841 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg
901 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
961 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
1021 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
1081 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
1141 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
1201 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
1261 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
1321 aattttaaca aaatattaac gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc
1381 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
1441 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
1501 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
1561 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
1621 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
1681 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
1741 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
1801 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
1861 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
1921 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
1981 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
2041 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
2101 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
2161 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
2221 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
2281 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
2341 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
2401 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
2461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
2521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
2581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
2641 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
2701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
2761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
2821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
2881 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
2941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
3001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
3061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
3121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
3181 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
3241 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
3301 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
3361 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
3421 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
3481 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
3541 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
3601 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
3661 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
3721 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
3781 cctagctcga tacaataaac gggtctctct ggttagacca gatctgagcc tgggagctct
3841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
3901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
3961 cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc
4021 agagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
4081 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
4141 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
4201 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
4261 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
4321 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
4381 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
4441 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
4501 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
4561 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
4621 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
4681 gcactatggg cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta
4741 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
4801 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
4861 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
4921 tgccttggaa tgctagttgg agtaataaat ctctggaaca gattggaatc acacgacctg
4981 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
5041 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
5101 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
5161 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
5221 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
5281 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
5341 agtgaacgga tctcgacggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
5401 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
5461 aattacaaaa attcaaaatt ttattccagt gtggtggaat tgagtattcc agtgtggtgg
5521 aattctgcag atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
5581 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
5641 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
5701 tccggctcgt ataatgtgtg gattttgagt taggatccgg cgagattttc aggagctaag
5761 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
5821 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
5881 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
5941 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
6001 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
6061 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
6121 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
6181 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
6241 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
6301 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat
6361 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
6421 ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
6481 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
6541 ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
6601 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
6661 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
6721 gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa atgcagttta
6781 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
6841 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
6901 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
6961 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
7021 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
7081 caggctccgt tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
7141 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
7201 tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatatcca gcacagtggc
7261 ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
7321 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
7381 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
7441 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
7501 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
7561 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
7621 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
7681 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
7741 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
7801 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
7861 ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
7921 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
7981 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
8041 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
8101 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
8161 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
8221 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
8281 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
8341 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accacactcc
8401 tgcatctgcc gccaccatgg cggaaggatc cgtcgccagg cagcctgacc tcttgacctg
8461 cgacgatgag ccgatccata tccccggtgc catccaaccg catggactgc tgctcgccct
8521 cgccgccgac atgacgatcg ttgccggcag cgacaacctt cccgaactca ccggactggc
8581 gatcggcgcc ctgatcggcc gctctgcggc cgatgtcttc gactcggaga cgcacaaccg
8641 tctgacgatc gccttggccg agcccggggc ggccgtcgga gcaccgatca ctgtcggctt
8701 cacgatgcga aaggacgcag gcttcatcgg ctcctggcat cgccatgatc agctcatctt
8761 cctcgagctc gagcctcccc agcgggacgt cgccgagccg caggcgttct tccgccgcac
8821 caacagcgcc atccgccgcc tgcaggccgc cgaaaccttg gaaagcgcct gcgccgccgc
8881 ggcgcaagag gtgcggaaga ttaccggctt cgatcgggtg atgatctatc gcttcgcctc
8941 cgacttcagc ggcgaagtga tcgcagagga tcggtgcgcc gaggtcgagt caaaactagg
9001 cctgcactat cctgcctcaa ccgtgccggc gcaggcccgt cggctctata ccatcaaccc
9061 ggtacggatc attcccgata tcaattatcg gccggtgccg gtcaccccag acctcaatcc
9121 ggtcaccggg cggccgattg atcttagctt cgccatcctg cgcagcgtct cgcccgtcca
9181 tctggaattc atgcgcaaca taggcatgca cggcacgatg tcgatctcga ttttgcgcgg
9241 cgagcgactg tggggattga tcgtttgcca tcaccgaacg ccgtactacg tcgatctcga
9301 tggccgccaa gcctgcgagc tagtcgccca ggttctggcc tggcagatcg gcgtgatgga
9361 agagtgagtc gacgcga
//
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